Which standard compound (eg: glycine, Lysine) can I use for analyzing Primary amine content in Polyethyleneimine using TNBS assay?

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Deon Bezuidenhout

Both should work, but perhaps if you had EDA (ethylenediamine) on hand, it would be useful to try as it is a very good small model compound for your PEI. Expect extinction coefficients in the region of 14000. Is your PEI linear of highly branched?

Santhosh Kalash Rajendrakumar

I am using 1.2K MW branched PEI,