Why does nanodrop show negative values for dye labelled ssDNA?

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How can I get a project topic and a research paper related to that topic?

I am a final year Msc. Applied Statistics student. For my project I need a topic and a research paper related to the topic. Please guide me.

26 October 2023 8,853 1 View

How to interpret EIS data of my perovskite solar cell?

The interpretation of the results of EIS is divided into two parts. on one hand, some researchers insist that larger semicircle is better because it leads to larger recombination resistance...

22 August 2023 7,742 2 View

What is the degassing condition to do BET surface analysis of Zn-BDC and Co-BDC MOFs ?

Zn-BDC and Co-BDC MOFs

31 July 2023 4,773 3 View

How to determine the final weights in fuzzy AHP at subclass level?

I have a basic understanding of the fuzzy AHP process. But there are a few confusion during the calculation of weights when calculated at the sub-class level. Example: I have 5 layers...

12 June 2023 4,879 0 View

Can someone please explain me the isolation procedure for bacterial protein from the supernatant (CFS) ?

I am actually trying to isolate bacterial lipopeptides from the supernatant. i tried isolating with 1) ethyl acetate and flash 2) acid precipitation and concentration explained in most of the...

14 April 2023 234 0 View

How to perform Water column correction on a satellite image?

I am working on coral bleeching process where I need to make water column correction on landsat images. I am not able to understand how do to perform this tech and using what software ? can it be...

08 April 2023 2,975 0 View

What may be the reason for foggy/whitish perovskite film after spincoating?

After spin coating, there will be a whitish or foggy perovskite film observed. in both one-step and two-step cases, it is happening. I cant get mirror like films. perovskite : triple cation...

20 March 2023 411 1 View

How the channeling form in alumina slurry ??

I have prepared Alumina slurry for dip coating at high pH using Darvon as a dispersant. I want to know how channeling form or reaction chemistry to the slurry formation.

12 January 2023 4,354 0 View

I would like to calculate the gas yield of a gas generating propellant. How the gas yield can be experimentally determined on a small scale level?

Method of determination of gas yield of a composite propellant.

18 December 2022 3,382 0 View

What is the recipe to prepare MOPS-KOH buffer?

I need a buffer for RNA studies which has both sodium and potassium ions. From some research papers I found that some labs have used MOPS-KOH buffer, but it is not available to buy when I searched...

03 November 2022 1,443 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View