I need a buffer for RNA studies which has both sodium and potassium ions. From some research papers I found that some labs have used MOPS-KOH buffer, but it is not available to buy when I searched for it. I hope I will get a solution from here. Thank you.
Juncen Guo
Hello! Have you ever gotten the recipe? I am also searching for it. Looking forward for your responding.
@Santra Santhosh
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Santra Santhosh
Hi, I finally got the recipe for MOPS-KOH. -->20mM MOPS (pH adjusted to 7 with KOH), 150 mM KCl and 10 uM EDTA. I got this from the following article.
Trachman, R.J., Autour, A., Jeng, S.C.Y. et al. Structure and functional reselection of the Mango-III fluorogenic RNA aptamer. Nat Chem Biol 15, 472–479 (2019). https://doi.org/10.1038/s41589-019-0267-9
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