Which spectroscopic/spectrometric technique can be used to fully identify compounds in a natural products mixture containing mainly phenolics and coumarins in the absence of LCMS?
Dear Sir. Concerning your issue about thespectroscopic/spectrometric technique can be used to identify mixtures of phenolics and coumarins. Phenolic compounds are well-known phytochemicals found in all plants. They consist of simple phenols, benzoic and cinnamic acid, coumarins, tannins, lignins, lignans and flavonoids. Substantial developments in research focused on the extraction, identification and quantification of phenolic compounds as medicinal and/or dietary molecules have occurred over the last 25 years. Organic solvent extraction is the main method used to extract phenolics. Chemical procedures are used to detect the presence of total phenolics, while spectrophotometric and chromatographic techniques are utilized to identify and quantify individual phenolic compounds. This review addresses the application of different methodologies utilized in the analysis of phenolic compounds in plant-based products, including recent technical developments in the quantification of phenolics.I think the following below links may help you in your analysis:
I have perused. I am doubting if the described method could help in the identification of specific phenolics/coumarins in a less complex mixture. At Adam, Could IR help since the compounds have similar overlapping functional group?