Using ethanol as the extraction solvent based on initial experiments. For dissolving DPPH, I am currently using methanol. Should I expect any differences if I were to use ethanol instead?
4o
The most suitable solvent for dissolving DPPH is typically ethanol itself in addition using the same solvent for both extraction and dissolution reduces the risk of solubility issues that mostly happen due to using different solvents. If ethanol concentration is too low or if there are other specific needs Consider DMSO as well. In addition, using Ethanol enhances DPPH solubility compared to methanol Maybe if possible you, conduct side by side comparison of DPPH activity in both solvents to evaluate any significant differences in your results especially if it could influence the radical scavenging activity.
DPPH is soluble in both ethanol and methanol, but using ethanol may enhance its solubility and stability. Switching solvents could lead to variations in the rate of DPPH reduction and antioxidant activity measurements.
The stock solution is made in DMSO . DMSO is miscible with methanol; or ethanol
Using the same solvent for both extraction and the DPPH assay ensures consistency and prevents solvent-related interactions that might interfere with the antioxidant activity measurement.
So, I suggest you use ethanol as the most suitable solvent in this case.
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