Hi, everyone.

I'm trying to detect p-TrkB, p-ERK and p-CREB in WB.

Which is better 5% skim milk or BSA for blocking?

I diluted two versions of antibody in TBST.

p-TrkB (1:1000 with 5% skim milk or with 5% BSA)

p-ERK (1:2000 with 5% skim milk or 5% BSA)

p-CREB (1:1000 with 5% skim milk or 5% BSA)

Thank you.