Which software can be used to find exon shuffling and gene fusion in transcriptome data?

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What is your major research area?

My major research area is clinical research and basic research of thyroid and parathyroid.

26 July 2024 3,639 14 View

Why did the simulated load-displacement curve of beam keep increasing and didn't decrease (ABAQUS)?

Dear colleagues, I'm currently modelling a simple 4-point bending test of a concrete beam in Abaqus. However, the load-displacement curve was ascending (without final point), which didn't match...

29 June 2024 6,192 2 View

How is conditioned medium collected? What is the significance of ultrafiltration for conditioned media?

Recently I've been doing some indirect co-culture experiments, in a nutshell, treating cancer cells with CAF's conditioned medium. So how do you do the collection of conditioned media and the...

01 June 2024 9,484 2 View

Is it correct to call model 2 (Hayes Process) a double moderation model?

I am using PROCESS (model 2) to conduct moderation analysis with two moderators (moderator W is a categorical variable while moderator Z is a continuous variable). DV is predicted by X (IV), W, Z,...

07 May 2024 8,042 0 View

What are the recent applications of chitosan in the field of environmental photocatalysis?

What are the recent applications of chitosan in the field of environmental photocatalysis? Have you ever implemented chitosan based environmental photocatalysts

06 May 2024 3,737 0 View

The ring compounds or polymers with diameters greater than 15nm?

Are there any ring compounds or polymers with diameters greater than 15nm that are easy to synthesize or cheap to purchase?

21 April 2024 9,131 0 View

Interaction terms in adonis2?

Hi, I'm new to bioinformatics. I have a weighted UniFrac metric generated from some bacterial samples and I want to test the effect of two factors A and B (both binary) on the composition using...

25 March 2024 9,619 1 View

Why is there no visible opalescent band formed after sucrose density gradient ultracentrifugation for phage purification?

I am currently conducting research on purifying Myoviridae phage particles using sucrose density gradient ultracentrifugation. However, I have encountered a challenge where no visible opalescent...

12 March 2024 9,387 3 View

Having both composite reliability and Cronbach's alpha above 0.6?

Composite reliability above 0.6, Cronbach's alpha above 0.6, and AVE above 0.5 are present simultaneously. Is this acceptable? Please share valid references to cite the same content." "Rho_c is...

06 March 2024 4,624 1 View

Digestion buffer causing loss of DNA yield on silica columns?

I am getting zero DNA yield after using qiagen purification columns. I finally traced the problem to NEBuffer 3.1, but pH doesn't seem to the cause. Essentially, I observe: 3 ug of DNA in 50 uL...

04 March 2024 5,861 6 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View