Ganesan Mahendran

5 Questions 3 Answers 0 Followers

Questions related from Ganesan Mahendran

Which region of the gene should I target for a CRISPR mediated Knockout of Target gene?

I want to design g RNA for knock out pathway gene to enhance secondary metabolites

30 July 2022 8,038 0 View

How to use CRISPOR software for design guide RNA?

I want to know tutorial help to design and how to choose suitable g for knok out

30 July 2022 4,058 2 View

How to prevent my RNA degradation?

I tried to isolate RNA from çatharanthus plant which contains more phenolic components. I couldn't isolation RNA. I used DEPC treated tips and all utilities and used Quigen kit, Trizol and isoplus...

24 March 2022 8,989 3 View

Can anybody Kindly suggest how to remove endophytic fungi during tissue culture?

I am trying to do Plant Tissue Culture with Bavistin, Benomyl and PPM to remove Endophytic Fungi. I used Bavistin, Benomyl at 200 mg/L and Soaking time is 1h and PPM at 1mg/L in culture medium,...

14 December 2015 567 9 View

How to control fungal infection in plant tissue culture?

I need procedures to control fungal contamination during tissue culture. I used systematic fungicide (Bavastin) at 0.5% for 2 hr at RT. I could not control fungal growth . Please suggest me how...

15 September 2015 7,475 21 View