Which protocol is best for nitrogen and phosphorus uptake from wastewater using microalgae?

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Is it possible to extract the Histones from the already isolated proteins by Np-40 Lysis buffer?

I need to do western blot against H3K9me3 and I tested it once from my Protein extracted by Np-40 lysis buffer, I had a very nice band but the problem is the band was not at the right size (15...

11 February 2024 5,692 0 View

Using the results of one simulation in the next simulation in COMSOL?

Hello I have generated a number of ions in COMSOL inside a chamber through the Electrostatic and Charged Particle Tracing modules (the first part of the simulation). Now I am going to move the...

17 July 2023 8,912 3 View

Modeling of Thermionic Emission in COMSOL (Freeman Ion Source)?

Hello I am going to simulate a Freeman ion source in Comsol. The Freeman ion source consists of a filament, which emits electrons by heating the filament. This filament is located in a...

07 July 2023 8,884 0 View

Can I solve a nonlinear force vibration issue under exponential force (Non periodic force), by use Method of Multiple scale (MMS) ?

I have a nonlinear ODE (second order) and exponential force in right side of equation ( q(t)=p0(1-t/t0)EXP[(-r*t)/t0] ) . p0, t0 and r are constant. I see in Nayfeh and Mook book only harmonic...

04 March 2021 5,201 4 View

Question about quantification of Aloe Vera polysaccharids formula?

I’m trying to follow your published protocol in article entitle “ Quantification of Aloe Vera polysaccharides by O-Acetyl and UV-Vis spectrophotometry: First Action 2018.14”. All parts are clear...

28 February 2021 6,109 5 View

How to extract polysaccharids from Aloe gel?

I 'm trying to extract polysacharides from Aloe leaf and tried ethanol percipitation, but after getting the pellet. I used the protocol from following reference following the steps, but I don't...

27 January 2021 7,332 5 View

How to visualise a journal issue?

Dear all, One of my friends is a editor in chief of a geospatial journal and he asked me to visualise the recent issue of the journal for publishing on the journal website. I have some ideas, for...

22 July 2020 6,500 4 View

Night light data for the city of Mashhad Iran?

Dear Colleagues, I am working on a project and need night light data for the city of Mashhad. I have searched on the internet and found that I could use the earth observatory website via this...

30 April 2020 370 0 View

Any recommendation for pathway analysis of Wheat RNA-seq data?

I have RNA-seq data for Wheat samples, and have a problem to find a way to do functional analysis such as Pathway analysis, any recommendation appreciated.

05 December 2019 5,235 11 View

What should we do if there are different kinds of study designs in a systematic review?

Dear All, Thank you for reading this question. I am conducting a systematic review of literature. The included articles have different study designs, for examples some of them are randomized...

29 November 2019 6,285 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

What roles do microorganisms play in Earth's ecosystems, & why is their role so important and role of microorganisms in secondary wastewatertreatment?

What roles do microorganisms play in Earth's ecosystems, and why is their role so important and role of microorganisms in secondary wastewater treatment?

23 July 2024 5,032 3 View

How to convert available phosphorus to total phosphorus?

I need to convert Available phosphorus into total phosphorus

18 July 2024 6,799 3 View

What could be the possible reason that sintering of polycrystalline diamond fabricated with tape casting has suddenly started failing?

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16 July 2024 3,212 5 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View

Asked a separate ways?

Dear all, I have a question regarding ways to separate ammonium nitrate and phosphorus pentoxide？

12 July 2024 3,164 2 View

J. C. Tarafdar

Dr. Kiani, the attached files may help you.

Mohamed S. Gaballah

I thank you need to read a lot about treatment using microalgea.

Khunsa Saeed Kiani

thank you sir J. C. Tarafdar .

Phanibhusan Ghosh

Micro algae normally prefers to uptake a fixed ratio of N/P. So, creation of suitable condition must be developed so that micro algae can easily uptake both these nutrients.