I’m trying to follow your published protocol in article entitle “ Quantification of Aloe Vera polysaccharides by O-Acetyl and UV-Vis spectrophotometry: First Action 2018.14”.
All parts are clear to me, but I’m not sure if I understand the total polysacharides calculation formula correctly:
As I’m working with callus, I scaled down the protocol and my starting material was 1 gr that I dissolved the extracted polysaccharids in 100 microliter of water and use all of it for quantification. My question is that in formula instead of “V” the total millimeters of sample solution, shall I put 0.1 ml or the total volume that I used for quantification after adding alkaline hydroxyl amine hydrochloride, HCL and ferric chloride which was 0.7 ml.
Total Aloe Vera polysaccharids (%, w/w)
=[(A - b) /m] x [ V /W} x100
A= the absorbance of the test solution at 540 nm
b = the y-intercept of the calibration curve
m= the slope of the calibration curve
V = the total millimeters of sample solution
W = the weight of sample in milligrams
Really appreciate your thoughts
Check and compare https://www.researchgate.net/publication/303594365_Main_Polysaccharides_Isolated_and_Quantified_of_Aloe_vera_Gel_in_Different_Seasons_of_the_Year
Also check https://www.researchgate.net/publication/7742192_Quantitative_colorimetric_analysis_of_aloe_polysaccharides_as_a_measure_of_Aloe_vera_quality_in_commercial_products
Chinaza Godswill Awuchi Thank you for sharing the articles, But I have already followed the methodology in the article that I mentioned
Article Quantitation of Aloe Vera Polysaccharides by O-Acetyl and UV...
And my question is for clarification of formula.
Thanks
I contacted the corresponding author and he said that for V, I have to use the volume that I dissolved the sample in, in my case (0.1 ml).
Please look at the following below links which may help you in your analysis.
Thanks
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