12 February 2024 0 6K Report

I need to do western blot against H3K9me3 and I tested it once from my Protein extracted by Np-40 lysis buffer, I had a very nice band but the problem is the band was not at the right size (15 KD) due to histone dimerized structure I could see the band at around 35 KD, now I would like to know if I can isolate the histones from my protein extract?

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