Should I use the covering of the follicle or the liquid fluid for RNA and protein extraction.
Which technique is easier to use
First, you must determine what proteins you are trying to extract. Second, you should look at research papers and review articles to find the protocols that others have used to extract the protein of interest. This will give you a start. You will try the various protocols and determine which one your protein of interest is extracted with maximum yield, minimal degradation.
Article Isolation of granulosa cells from follicular fluid; applicat...
This link may help
Hi Collin, please check this paper for RNA follicle extraction protocol from granulosa cells.
Wang, Y., Li, J., Ying Wang, C., Yan Kwok, A. H., & Leung, F. C. (2007). Epidermal growth factor (EGF) receptor ligands in the chicken ovary: I. Evidence for heparin-binding EGF-like growth factor (HB-EGF) as a potential oocyte-derived signal to control granulosa cell proliferation and HB-EGF and kit ligand expression. Endocrinology, 148(7), 3426-3440.
and this one.
Gilbert, A. B., Evans, A. J., Perry, M. M., & Davidson, M. H. (1977). A method for separating the granulosa cells, the basal lamina and the theca of the preovulatory ovarian follicle of the domestic fowl (Gallus domesticus). Reproduction, 50(1), 179-181.
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