What is the best way for synthesis of 2',4-dihydroxychalcone?

I'm trying to synthesize 2',4-dihydroxychalcone using 2-hydroxyacetophenone and 4-hydroxybenzaldehyde. I've done several sythesis but all I get was some kind of oil and no precipitate of product...

03 April 2019 6,167 4 View

Can you use a t-test instead of an ANOVA in a multi-factorial design if you're interested in only one comparison?

25 February 2019 9,243 4 View

Does HRV-3C protease cleave TEV cleavage site?

I have heard some rumors that the HRV-3C protease can recognize and cleave ENLYFQG peptide, which is a consensus sequence cleaved by the TEV protease. Can anyone confirm or suggest some literature...

23 July 2018 1,321 4 View

Are there any wide-spectrum kinase inhibitors?

I wanted to analyze the phosphorylation of a certain mammalian protein. It is a standard procedure to add phosphatase inhibitors to extraction buffers to block the process of dephopshorylation....

12 March 2018 8,978 4 View

What book/article would be a good introduction into artificial neural networks?

Hi, I'd like to learn more about artificial neural networks, specifically pattern recognition algorithms. Unfortunately, I don't have much of a background in mathematics, so I'm looking for...

26 December 2017 4,850 2 View

Best tool for generating topology of polymer brushes (MD)?

17 September 2017 7,160 1 View

What is the resolution of ion-exchange chromatography?

Dear all, I would like to ask what is the maximal achievable resolution of ion exchange chromatography in the matter of pI values. In particular I am wondering whether I will be able to separate...

11 October 2016 5,605 3 View

Software for load path optimized structural design of fiber reinforced materials?

Which software would be most suitable for carrying out a load path analysis of an existing part, such as an aircraft wing structure, and subsequently doing a topology optimization of that design...

09 August 2016 874 1 View

What are the best 3 different reporter proteins as fusion partners for structural studies?

07 July 2016 5,040 2 View

Can someone provide me with some advice on using BS3 crosslinker and DTT buffer?

I would like to perform a crosslinking experiment using BS3 crosslinker. My protein has 3 cysteines, but no S-S bridge, so I have to keep my protein in buffer with 5mM DTT. Can I perform the...

16 July 2013 9,690 9 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View