Hi everyone!
I have a problem with slime in my protein sample. For isolation, I use RIPA buffer + protease inhibitors.
When I measured the protein concentration immediately after isolation everything was ok.
The problem appeared when I dissolved the samples the next day. I noticed slime and the protein concentration dropped dramatically. I assume that the culprit is the DNA.
To solve this problem I would like to supplement the RIPA buffer with DNAse I, is this feasible? If so, what concentration would be best? 10 ug/ml?
Supplementing your RIPA buffer with DNAse I to address the slime issue caused by DNA contamination is feasible. A concentration of 10 ug/ml is commonly used for DNAse I treatment in protein samples. However, it's essential to optimize the concentration based on your specific sample and experimental conditions. You may need to perform a titration experiment to determine the optimal DNAse I concentration that effectively removes the DNA without compromising protein integrity. Start with 10 ug/ml and adjust as needed based on the results of your experiment.
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