What is the best way for synthesis of 2',4-dihydroxychalcone?

I'm trying to synthesize 2',4-dihydroxychalcone using 2-hydroxyacetophenone and 4-hydroxybenzaldehyde. I've done several sythesis but all I get was some kind of oil and no precipitate of product...

03 April 2019 6,167 4 View

Can you use a t-test instead of an ANOVA in a multi-factorial design if you're interested in only one comparison?

25 February 2019 9,243 4 View

Does HRV-3C protease cleave TEV cleavage site?

I have heard some rumors that the HRV-3C protease can recognize and cleave ENLYFQG peptide, which is a consensus sequence cleaved by the TEV protease. Can anyone confirm or suggest some literature...

23 July 2018 1,321 4 View

Are there any wide-spectrum kinase inhibitors?

I wanted to analyze the phosphorylation of a certain mammalian protein. It is a standard procedure to add phosphatase inhibitors to extraction buffers to block the process of dephopshorylation....

12 March 2018 8,978 4 View

What book/article would be a good introduction into artificial neural networks?

Hi, I'd like to learn more about artificial neural networks, specifically pattern recognition algorithms. Unfortunately, I don't have much of a background in mathematics, so I'm looking for...

26 December 2017 4,850 2 View

Best tool for generating topology of polymer brushes (MD)?

17 September 2017 7,160 1 View

What is the resolution of ion-exchange chromatography?

Dear all, I would like to ask what is the maximal achievable resolution of ion exchange chromatography in the matter of pI values. In particular I am wondering whether I will be able to separate...

11 October 2016 5,605 3 View

Software for load path optimized structural design of fiber reinforced materials?

Which software would be most suitable for carrying out a load path analysis of an existing part, such as an aircraft wing structure, and subsequently doing a topology optimization of that design...

09 August 2016 874 1 View

What are the best 3 different reporter proteins as fusion partners for structural studies?

07 July 2016 5,040 2 View

Which one of databases do you recommend to explore validated targets for miRNA categorized by disease/metabolic disorder?

24 November 2014 8,772 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View