What are some qualitative research analytical methods that seem to be aligned and a good fit for research that prioritizes a social justice lens?

I'm a qualitative researcher myself. I'm familiar with a couple of analytical methods (some very popular ones and a few not so popular ones) such as: Thematic analysis Qualitative content...

01 December 2020 3,478 5 View

What is the suitable tamping method for ladder-sleeper ballasted tracks?

Since the ladder sleeper consists of two longitudinal beams along the railway track, a conventional tamping machine is not applicable for ladder tracks.

26 November 2020 3,172 1 View

Which centrifugal dna concentrator is better for double strand DNA ( microcon, centricon or vivacon)?

We need to concentrate DNA after extraction. Using concentrators is a normal way. Which one among centricon, microcon and vivacon filters make more DNA concentration.

19 July 2020 5,356 2 View

Natural Frequencies of doubly clamped beam?

Hello, I have a doubly clamped nano-beam and I want to obtain its natural frequencies for: 1- Low stress and 2- High stress using COMSOL software. How can I involve the impact of the stress level...

17 June 2020 3,244 3 View

Casting of H11 and H13 tool steels?

Are investment casting and squeeze casting the only techniques to cast tool steels, H11 and H13? If so, what is the reason?

17 December 2019 4,653 1 View

Is a dataset consisted of 25000 samples large for meta-analysis? If so, are the p-value and confidence interval reliable to interpret the results?

Hi, Considering the student's t-test formula, when sample sizes are large, it's easier to find significant p-values when they're not really there (i.e., false positives), because significance gets...

28 October 2019 2,452 7 View

Is there any method to extract microRNA from blood samples without using commercial kits?

Hi everyone, I am seeking a method to extract miRNA from blood samples without using commercial kits. Any help would be appreciated.

14 September 2018 7,656 3 View

I`m looking for the reasons why my recombinant protein in Pichia Pastoris have expressed well in shaked flask BUT It`s very low in fermenter?

I`ve checked all of the key parameters for scale up(PH-Do-Feed rate...)

03 February 2018 602 2 View

Is it possible to calculate the value of dislocation density quantitatively using GND (geometry necessary dislocation) MAP?

29 April 2017 9,131 4 View

Is It possible to determine dislocation density of poly-crystalline Ti64 alloy using ATOM software? what kind of file is necessary for this?

EBSD analysis of samples is available. is it possible to import EBSD file to ATOM for dislocation density determination?

16 April 2017 9,412 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View