Yurii V Geletii Sir, In the equation (y=mx+b), I put the absorbance of my plant sample in place of y and calculate the value of X. Then this value of X is put in the formula TFC= CV/M.
You should use the type of flavonoids that you are expecting in your samples, it could be Quercetin, Rutin, or Catechin.
Dear Preety Rohilla thank you for your interesting technical question. Yes, you can use quercetin as the standard for flavonoid assay. Please see e.g. the following useful article:
Assessment of Total Phenolic and Flavonoid Content, Antioxidant Properties, and Yield of Aeroponically and Conventionally Grown Leafy Vegetables and Fruit Crops: A Comparative Study
Article Assessment of Total Phenolic and Flavonoid Content, Antioxid...
This paper is freely available as public full text on ResearchGate. It is stated in this work that "For total flavonoid determination, quercetin was used to make the standard calibration curve."
The AlCl3 assay has been actually developed by
Lamaison, JL; Carnet, A (1991). "Teneurs en principaux flavonoides des fleurs de Cratageus monogyna Jacq et de Cratageus Laevigata (Poiret D.C) en Fonction de la vegetation". Plantes Medicinales Phytotherapie. 25: 12–16.
Quercetin was used as a standard
Sir, Yurii V Geletii & Frank T. Edelmann Abul Hossain, Thanks all of you for your response.
Frank T. Edelmann Thank you, Sir, for providing this useful article.
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