Abul Hossain

13 Questions 16 Answers 0 Followers

Questions related from Abul Hossain

How can I determine the final concentration of an extract after concentrating by a rotary evaporator?

I've added 20 ml methanol with 1 g powder then it was evaporated by a rotary evaporator and re-dissolved in 4 ml methanol and then it was diluted with 10 ml water. So, How can I calculate the...

31 October 2017 6,605 4 View

How can I prepare 10ml of Tris–HNO3 buffer (pH 8.2, 1%NaCl)?

I am a little bit confused about Tris-HNO3 buffer (pH 8.2, 1%NaCl). I have only HNO3 and NaCl reagent available. However, Tris and buffer words make me confused. In this case, I need to be more...

20 April 2017 2,189 3 View

How many times Immunomagnetic beads (IMBs) can be possibly used without any further coupling with antibody solution to the beads?

This is my new project research work upon "microorganism isolation from food sample using nanomagnetic particles". Briefly, first of all, MBs(magnetic beads) were coupled with anti-Yersinia...

02 January 2017 5,774 3 View

How can I prepare protein (peanut allergen) sample to observe under TEM?

I have done one time TEM experiment to observe the peanut protein(Ara h1).The process was simply with 3 microlitre protein sample diluted into 1 ml PBS(Buffer) and then a drop of diluted sample...

21 December 2016 9,084 5 View

Are conjugated Dynabeads with Yersinia antibody commercially available for IMS method?

My research is based on separation of Yersinia enterocolitica from raw untreated milk by using magnetic beads or dynabeads following Immunomagnetic separation method (IMS). If any knows about...

29 August 2016 8,649 1 View

What is a good value of R²?

I am trying to find total phenol from persimmon leaves but in a standard curve, the values of gallic acid show R²=0.9025 (y = 23.75x - 33.75). Is it acceptable?

09 August 2016 7,216 3 View

I would like to know that which size of dyna-magnetic beads are preferable for immunomagnetic cell separation?

Recently I am going to begin research on IMS to separate Yersinia enterocolitica cells from food sample.In this case, to design experimental protocol, i need correct information about Dyna...

18 July 2016 1,373 0 View

What is the best way to make SEM sample preparation( Bacteria) on SWCNTs based biosensor?

I am trying to get SEM result for Yersinia enterocolitica from SWCNTs based biosensor.However I am not getting expected result. Procedure was.. 1) CNT immobilization 2)Linker...

08 July 2016 526 2 View

What is the best way to wash microplate assay for ELISA experiment?

Dear brothers and friends What is the best way to wash microplate assay for ELISA experiment? how much time can I take to melt PnPP Substrate in buffer solution?

15 June 2016 8,591 1 View

How do I determine the reducing sugar percentage from wine?

sample: grape wine

07 April 2016 6,896 7 View

How much sulfur dioxide used to preserve food in various countries?

Permitted concentration of SO2 for preserving food specially in dry fruits in different countries.

17 March 2016 289 3 View

How can I make 3.8 ml ethanol solution of DPPH radical (final concentration was 0.1 mM)?

How can I make 3.8 ml ethanol solution of DPPH radical (final concentration was 0.1 mM) to determine Radical DPPH Scavenging Activity?

03 February 2016 4,705 2 View

How can I measure the impedance by using potentiostat?

I am working on the development of SWCNTs-based biosensor for rapid detection of bacteria. I would like to measure the interactions caused by the binding of antibody-antigen upon the biosensor...

01 January 1970 3,710 3 View