for methanolic extract is Dioscorea bulbifera
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...
01 March 2021 1,843 2 View
I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...
23 February 2021 5,167 3 View
I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...
20 February 2021 6,058 2 View
I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...
11 February 2021 6,852 4 View
I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...
10 February 2021 6,145 3 View
I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...
02 February 2021 8,806 2 View
Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...
31 January 2021 4,271 3 View
I started learning the Western Blot a few days ago and I have an okay understanding of the process, but there are a few steps I don't understand how to do by myself from scratch. I want to...
25 January 2021 6,691 8 View
Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...
25 January 2021 8,333 3 View
