Can any one tell me the indirect method to calculate SOD based on Quercetin?

Prachi Mistry @Prachi-Mistry-5

09 March 2024 0 6K Report

Full method and formula to calculate SOD based on Quercetin in animal tissue

Badges
Science topic

Similar topics
Biological Science
Histology

More Prachi Mistry's questions See All

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How are Large Models Exploring and Outputting Knowledge Understanding in Specific Content Areas, and What Does Academic Research Say About It?

Hello everyone！ I am currently exploring the performance of large models in understanding knowledge in specific domains, and attempting to construct a knowledge framework similar to what...

05 August 2024 5,729 2 View

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity?

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...

03 August 2024 5,358 0 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

Is artifacts in XPS possible to build high deviation in binding energy larger than 5 eV??

Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...

30 July 2024 9,824 2 View

Which statistical test should we use?

N=6 Comparing pre and post test likert scale responses. Participants are mix of practicing & preservice teachers.

30 July 2024 7,233 4 View

How to build my own lab made four point probe set up?

Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...

30 July 2024 906 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

If my standard quercetin equation is Y = 0.0057x + 0.0635 then how can I know the value of ethanolic extract of 10 mg/ml if absorbance value is 0.39?

I wanted to know the value of total flavonoid content in mgQE/gm. Total volume I kept in well plate is 200 µl in which extract was 20 µl. Anyone please help me with this problem

10 May 2024 5,658 4 View

Why SOD of samples are higher than control?

Hello I'm measuring SOD activity in plant leaves using NBT method. Reaction solution contains EDTA, riboflavin, methionine and PBS. But my samples values are higher than control. Do you know how I...

09 May 2024 8,930 1 View

Kit for SOD assay in Plants?

Can anyone suggest any kit for SOD activity in plant? I have the protocol for NBT reduction but don't have centrifuge for falcon as the protocol requires 3mL of homogenate to be centrifuged in...

06 May 2024 1,411 4 View

What is the best solvent system for quercetin ,kaempferol and diosgenin quantification ?

for methanolic extract is Dioscorea bulbifera

21 April 2024 8,349 0 View

Creating oxidative stress model in cell culture?

Hello everyone! MTT was performed after applying DEHP to HeLa cells. According to MTT results (24 hours of exposure), cell viability is significantly reduced at concentrations of 100 micromolar...

27 February 2024 503 2 View

Can you use the same sample prep buffer for the GSH/GSSG assay and SOD assay?

I have ordered the SOD colorimetric kit (invitrogen) and a GSH/GSSG ratio assay (abcam, ab205811) and was hoping to use the same brain tissue for both kits. In the methods provided the by the...

11 February 2024 4,390 2 View

Can we measure Superoxide dismutase activity in dried leaves?

I have dried leaves from my past experiment and need to conduct SOD activity assay. Can it be done in dried leaves?

19 January 2024 7,281 4 View

Can I calculate co-efficient value of WST formed in superoxide dismutase enzyme ?

I wanted to find out the unit of my nanoparticle"s SOD activity.

13 November 2023 5,815 0 View

Amount of Plasma needed for Antioxidant analysis (SOD, GSH-Px, MDA) for Dairy Cow's?

What is the total amount of plasma required for analysis of plasma SOD, GSH-Px, and MDA in dairy cattle? Provided references if possible.

12 November 2023 7,219 0 View