I prepared the nutrient broth and revive the bacteria (C.V) using the stock culture of C.V. The culture was 10-15 days old and bacteria revived in the broth.

After 25 days, I tried to revive the bacteria on the nutrient agar plate by streaking method but no growth was observed on the agar plate. Then I tried to revive the bacteria in broth from the same stock culture but there was no bacterial growth in the broth also.

But on the stock culture plate, there is good growth of bacterial colonies. So what could be the probable reason that bacteria not revived in the broth as well as nutrient agar?

Kindly share your suggestions.

Thanks