How does the gelling medium play a role in supply of nutrients to the ex-plant?
It depends on the specific application. Both of them have advantages and disadvantages. This is from Plant Cell Culture Protocols, Methods in Molecular Biology vol. 111:
Agars and agaroses generally produce gels that are stable for prolonged periods and are considered not to bind media components excessively. Products with various degrees of purity are available, and low-gelling temperatures types can even enable embedding of sensitive cells, such as protoplasts. On the other hand, Gelrite/phytagel produces a rigid gel at much lower concentrations than agar or agarose. They are also almost transparent, which makes it easier, e.g., to identify contamination at an early stage. These gels do, however, tend to liquify in long-term cultures owing to pH changes or the depletion of salts necessary for crosslinking. Higher concentrations of antibiotics (e.g., kanamycin) may also be required in Phytagel/gelrite solidified media in comparison to those solidified with agar/agarose.
The only thing I heard is that with Phytagel one can have problem with Kanamycin selection. But I have no personal experience with it.
Phyta gel is better as we have seen the results in rice plantlet regeneration
Phyta gel is better as more clear/transparent media preparation is possible
It depends on the specific application. Both of them have advantages and disadvantages. This is from Plant Cell Culture Protocols, Methods in Molecular Biology vol. 111:
Agars and agaroses generally produce gels that are stable for prolonged periods and are considered not to bind media components excessively. Products with various degrees of purity are available, and low-gelling temperatures types can even enable embedding of sensitive cells, such as protoplasts. On the other hand, Gelrite/phytagel produces a rigid gel at much lower concentrations than agar or agarose. They are also almost transparent, which makes it easier, e.g., to identify contamination at an early stage. These gels do, however, tend to liquify in long-term cultures owing to pH changes or the depletion of salts necessary for crosslinking. Higher concentrations of antibiotics (e.g., kanamycin) may also be required in Phytagel/gelrite solidified media in comparison to those solidified with agar/agarose.
I have used both before in wheat and barley. Phytagel works much better than agar-agar.
In invitro screening for Striga resistance, can I use phytagel instead of agar?
it spends on the superpose of your experiment. Agar is known as the best gelling agent, however in some cases phytagel used for its transparency and cheaper price.
Gustavo Acevedo-Hernandez : could you please let me know the full citation details of this interesting article in "Plant Cell Culture Protocols, Methods in Molecular Biology vol. 111:"
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