18 Questions 11 Answers 0 Followers
Questions related from Jimmy John Lilly
I have two questions: The binding energy of complex is negative. Why? can u suggest any tools to determine the interacting energies for a docked complex?
07 July 2018 10,090 0 View
I need to know the best absorbance value that correlates the growth of Agrobacterium that can be used to infect plant callus. I have tried with 1.0 at 600 nm infection which helped me to transform...
02 February 2018 931 1 View
I need to find MoleculeInteraction energyVander Waals energyElectrostatic energyHydrogen bond energySolvation free energyInteracting energy I have already tried autodoc since the ligand is a...
07 July 2017 4,669 2 View
I get contamination either fungal or bacterial after a two week successful establishment of Plant callus. Please tell me why it happens? Endophytes are ruled out totally since even after the...
01 January 2016 5,475 11 View
why is it that only E.coli BL21(DE3) strain is widely used for eukaryotic gene expression when other strains can also be used. what is the full form of BL21(DE3). I searched but i did not find...
09 September 2015 4,419 4 View
I want to know how to use map Inspect software. I tried searching publications related to Map Inspect protocol. but I was unable to find anything about the same. I need to know the...
05 May 2015 9,738 1 View
How to determine calorimetrically? HPLC methods exist but can one determine using UV spec or fluorometry?
10 October 2014 1,981 3 View
What is the probable mechanism of WRKY Transcription factor or the pathway in which wrky plays a major role against biotic stress. Please provide references.
09 September 2014 6,270 7 View
Need to know new methods which can help in proving the role of a particular gene in a specific function of the gene.
06 June 2014 3,625 2 View
I get no spots in my 2D gel. Can not fixing it be an answer? I have tried everything but I cannot seem to fix this problem. Please advise.
05 May 2014 2,790 10 View
please help me by providing the protocol for entire PLANT proteins
05 May 2014 479 3 View
I need to see the introns and exons of the specific gene sequence of plants. I would like to know how exactly to use them too.
03 March 2014 5,157 2 View
I'd like to know more related to plant immunity and plant resistance pathways.
01 January 2014 9,616 2 View
Mature ex-plants have lot of hair resulting in fungal contamination. Even after using 1% sodium hypochlorite and 70% ethanol, it doesn't work.
11 November 2013 7,318 7 View
How does the gelling medium play a role in supply of nutrients to the ex-plant?
10 October 2013 1,361 9 View
I have used internode and leaf, but even after increasing the time of sterilization, it is getting contaminated. I only got a minute callus from a leaf disc. What should i do to preserve it? What...
10 October 2013 4,935 8 View
Is there any harmful effect on the growth of the explant ?
09 September 2013 3,750 83 View
Where can i find the literature for casein hydrolysate and its specific role?
09 September 2013 2,543 4 View
