I have two questions: The binding energy of complex is negative. Why? can u suggest any tools to determine the interacting energies for a docked complex?
I need to know the best absorbance value that correlates the growth of Agrobacterium that can be used to infect plant callus. I have tried with 1.0 at 600 nm infection which helped me to transform...
01 February 2018 959 1 View
I need to find MoleculeInteraction energyVander Waals energyElectrostatic energyHydrogen bond energySolvation free energyInteracting energy I have already tried autodoc since the ligand is a...
06 July 2017 4,693 2 View
I get contamination either fungal or bacterial after a two week successful establishment of Plant callus. Please tell me why it happens? Endophytes are ruled out totally since even after the...
31 December 2015 5,509 11 View
why is it that only E.coli BL21(DE3) strain is widely used for eukaryotic gene expression when other strains can also be used. what is the full form of BL21(DE3). I searched but i did not find...
08 September 2015 4,472 4 View
I want to know how to use map Inspect software. I tried searching publications related to Map Inspect protocol. but I was unable to find anything about the same. I need to know the...
04 May 2015 9,766 1 View
How to determine calorimetrically? HPLC methods exist but can one determine using UV spec or fluorometry?
09 October 2014 2,027 3 View
What is the probable mechanism of WRKY Transcription factor or the pathway in which wrky plays a major role against biotic stress. Please provide references.
08 September 2014 6,317 7 View
Need to know new methods which can help in proving the role of a particular gene in a specific function of the gene.
05 June 2014 3,673 2 View
I get no spots in my 2D gel. Can not fixing it be an answer? I have tried everything but I cannot seem to fix this problem. Please advise.
04 May 2014 2,864 10 View
please help me by providing the protocol for entire PLANT proteins
04 May 2014 506 3 View
How to develop investments in renewable energy sources?
08 August 2024 5,112 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...
30 July 2024 9,824 2 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View