What is the best absorbance value of Agrobacterium culture to be used to infect plant callus?

I need to know the best absorbance value that correlates the growth of Agrobacterium that can be used to infect plant callus. I have tried with 1.0 at 600 nm infection which helped me to transform...

01 February 2018 863 1 View

I carried out patchdoc for Protein-DNA and got the result but i need a tool for find interaction energy

06 July 2017 4,604 2 View

Why do I get contaminations after two-three weeks sucessful plant callus growth?

I get contamination either fungal or bacterial after a two week successful establishment of Plant callus. Please tell me why it happens? Endophytes are ruled out totally since even after the...

31 December 2015 5,403 11 View

Why is E.coli BL21(DE3) strain used in eukaryotic expression?

08 September 2015 4,297 4 View

How can I use the software "Map Inspect" to find locations of genes?

I want to know how to use map Inspect software. I tried searching publications related to Map Inspect protocol. but I was unable to find anything about the same. I need to know the...

04 May 2015 9,668 1 View

How can one estimate Salicylic acid and Jasmonic acid in plants using calorimetry?

How to determine calorimetrically? HPLC methods exist but can one determine using UV spec or fluorometry?

09 October 2014 1,901 3 View

What is the mechanism of WRKY transcription factor against biotic stress?

What is the probable mechanism of WRKY Transcription factor or the pathway in which wrky plays a major role against biotic stress. Please provide references.

08 September 2014 6,141 7 View

What are the new advanced techniques in plant molecular biology involved in proving disease resistance?

Need to know new methods which can help in proving the role of a particular gene in a specific function of the gene.

05 June 2014 3,523 2 View

Can fixing the gel be a problem for no spots in your 2D gel?

I get no spots in my 2D gel. Can not fixing it be an answer? I have tried everything but I cannot seem to fix this problem. Please advise.

04 May 2014 2,693 10 View

Why don't I get spots in 2D gel even after a proper isoelectric focussing?

please help me by providing the protocol for entire PLANT proteins

04 May 2014 413 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View