What is the reason that the binding energy of a docked complex is negative?

I have two questions: The binding energy of complex is negative. Why? can u suggest any tools to determine the interacting energies for a docked complex?

06 July 2018 9,987 0 View

What is the best absorbance value of Agrobacterium culture to be used to infect plant callus?

I need to know the best absorbance value that correlates the growth of Agrobacterium that can be used to infect plant callus. I have tried with 1.0 at 600 nm infection which helped me to transform...

01 February 2018 863 1 View

I carried out patchdoc for Protein-DNA and got the result but i need a tool for find interaction energy

06 July 2017 4,604 2 View

Why do I get contaminations after two-three weeks sucessful plant callus growth?

I get contamination either fungal or bacterial after a two week successful establishment of Plant callus. Please tell me why it happens? Endophytes are ruled out totally since even after the...

31 December 2015 5,403 11 View

Why is E.coli BL21(DE3) strain used in eukaryotic expression?

08 September 2015 4,297 4 View

How can I use the software "Map Inspect" to find locations of genes?

I want to know how to use map Inspect software. I tried searching publications related to Map Inspect protocol. but I was unable to find anything about the same. I need to know the...

04 May 2015 9,668 1 View

How can one estimate Salicylic acid and Jasmonic acid in plants using calorimetry?

How to determine calorimetrically? HPLC methods exist but can one determine using UV spec or fluorometry?

09 October 2014 1,901 3 View

What is the mechanism of WRKY transcription factor against biotic stress?

What is the probable mechanism of WRKY Transcription factor or the pathway in which wrky plays a major role against biotic stress. Please provide references.

08 September 2014 6,141 7 View

Can fixing the gel be a problem for no spots in your 2D gel?

I get no spots in my 2D gel. Can not fixing it be an answer? I have tried everything but I cannot seem to fix this problem. Please advise.

04 May 2014 2,693 10 View

Why don't I get spots in 2D gel even after a proper isoelectric focussing?

please help me by providing the protocol for entire PLANT proteins

04 May 2014 413 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View