Anyone who can help me with this question?
a. Wash the plate several times with fresh medium previously filtered through a 0.4 ?m filter.
b. Wash the plate several times with fresh medium previously filtered through a 0.2 ?m filter.
c. There is not any way for efficiently and permanently eradicating Mycoplasma from a tissue culture once it is contaminated.
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For avoiding contaminations including mycoplasma, you need to decontaminate all the facility of your culture and can use ready to use media from commercial supplier. In addition, You can eradicate mycoplasma from your cells using commercial kits.
Good Luck!
Dear efshan
you van try to treat your cells with appropriate antibiotics effective against micoplasma https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3470117/
the only way to protect medium from micoplasma contamination is the filtration at 0.1um ( 0.22 and 0.4 are not effective) but this is able to prevent contamination and not to solve it
good luck
Manuele
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