There are many types of systems in the bacteriological laboratory are used for identification of bacteria Pseudomonas spp. which one is gratified.
Once you isolated bacteria on Pseudomonas Oxford selective agar, identification can be easier with API-Pseeudomonas Kits and follow the instructions listed in the brosure enclosed.
There are currently bacteriologically isolated organisms that are directly and easily isolated, such as the chromoagar media. And API good for specific isolates of Pseudomonas
Vitek is a really good system to identify several Psedumomas members. Easy, automated, efficient. Really good.
hi
can you use the two system for pseudomonas identifican but I'd prefer vitek system because it's give you the very good identification
and now I used the two system for identification
Both API as well VITEC can use for the Identification of Pseudomonas species. VITEC is automated system to identify the bacteria.
Both the systems are good for bacterial identification even if the Vitek system is better especially for environmental species; moreover you have to consider the cost that for ViteK is much higher than for the API system.
I prefer API ( API 20 NE) because all my results were consistent to results got from molecular data when we compared two methods. But it is a very important note-we check bacteria in the fish farming
Vitek II is more accurate automated system for identification but as regard to the cost you can use the API.
obviously Vitek-2 Compact system will be the ultimate choice as this system is automated and accuracy is high. However considering the cost effectiveness, you can choose API 20 NE after screening the isolates with selective media (PCA) and Oxidase test kit.
Pseudomonas is the species with simple id. API costs 4 $, Vitek 12 $.
Vitek gives additionally susceptibility.
Wojtek
Majority of Pseudomonas isolated in clinical setting are P. aeruginosa. Vitek does very well with identification and performing susceptibility testing. However when it comes to other Pseudomonas species Vitek is not so reliable. Non-fermentor API are much better with regard to this last category. However it is more labor intensive.
Vitek has a wide data base. May be you can complement with other biochemistries
In the case of environmental strains of pseudomonads biochemical testing can be tricky. Probably the best way to make the identification is to amplify and read sequences coding rRNA (e.g. 16S rRNA). In our case, API indicated Burkholderia cepacia and in fact it was Pseudomonas aeruginosa. We confirmed it with PCR-based methods.
I prefer API ( API 20 NE) because all my results were consistent to results
Adrian, wystarczy zrobić antybiogram i się przekonasz jaki to gatunek. Ale wiedzę, że oni robią badania mikrobiologii środowiskowej, a nie prawdziwej, czyli medycznej.
Wg wzorów oporności dojdziesz co to za syf w wodzie :)
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