What is the difference between an oligonucleotide primer that used in PCR assay than that used in DNA Sequencing?

Oligonucleotide primers are used to generate bands corresponding to their own gene in PCR assay.

06 September 2020 2,066 7 View

Arabic essay grading dataset?

Can you help me find an arabic essay grading dataset to be used for machine learning?

15 April 2019 3,998 3 View

What are the primer sequences used for APRIL-mRNA expression by RT-PCR?

Can anybody have the correct primer sequences to amplify APRIL gene (TNF-family) for reverse transcription of m-RNA as different articles use different primer sequences as well as the primers for...

26 March 2019 1,494 3 View

How can I judge my time domain measurment accuracy?

Model testing was conducted and we have measurements which varies with time of course such as (speed vs time), (motor current vs time), (force vs time), etc. I just want to measure the accuracy of...

08 January 2019 153 3 View

After submitting a revised manuscript (moderate revision was requested), what are the steps followed ?

15 December 2017 140 90 View

In a set of connected Nodes(routers), how can i measure the distance between them ?

In a set of connected Nodes(routers), how can i measure the distance between them in which i can predict how much time a Packet take to reach next node? Thank You.

06 April 2017 726 8 View

How to register Somatosensory Evoked Potentials (SEPs) using CED Signal software?

I was thinking of recording SEPs in patients with movement disorders. We are using CED signal software in our lab to register MEPs. It has been stated on the software website that it can be used...

28 January 2017 5,897 1 View

How can we estimate some missing values of a spring water flow?

12 August 2016 4,174 10 View

I want to ask about the practical way to transfer N fixing genes between different microorganisms ?

02 February 2016 4,961 2 View

I'm looking for induction machine thermal model using MATLAB, can any one help me with that?

The important indicator on the electrical machine status is the temperature of the equipment itself. Many mechanical faults and sometimes electrical faults manifest themselves as equipment...

10 June 2014 3,522 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View