Most of the studies use primary cells or stem cells for modelling the spinal cord, but as I never worked with these cells, I would like to start with a cell line. Can anybody suggest a good microglial cell line?
Thanks in advance!
Hello,
Most microglial lines have been used as inflammation models.
The best known mouse microglial line is BV-2 which is easy to culture (similar to RAW264.7 cells in requirement but even easier to manage). However, BV-2 seems to remain commercially unavailable. You may need to write and request or ask around a nearby lab that has a stock of it.
For culture details, see SI of p. S15-16.
http://www.rsc.org/suppdata/c5/sc/c5sc03855c/c5sc03855c1.pdf
Recently a mouse microglial line has become accessible with ATCC.
SIM-A9 (ATCC® CRL-3265™); https://www.atcc.org/products/all/CRL-3265.aspx
So is a human microglial line: HMC3 (ATCC® CRL-3304™). https://www.atcc.org/Products/All/CRL-3304.aspx#generalinformation
Good luck.
Hi, thank you for your input.
I was looking around for providers of the BV-2 cell line, and you are right it seems is not commercially available.
I had found this SIM-A9 in ATCC website and also they have this C8-B4 cell line, both are mouse microglial line, have you worked with any of them or have any suggestion for choosing between these two cell lines?
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