I would like to do a co-culture of microglia and astrocytes for making a model of spinal cord inflammation. We have some concerns regarding compatibility between them. We want to be sure they can grow together in a culture.
Can anybody suggest good microglial and astrocytes cell lines for that?
Primary microglial or astrocyte cell culture has to be prepared by isolating these cells from prefrontal cortex of neonatal wistar rats or mouse. One can follow the protocol briefly described in the article titled "Microglia isolation from adult mouse brain (2014) et al., Jae-Kyung Lee".
Primary rat cortical cells which maintained in a serum-free culture media could be useful for making a co-culture.
Hi Estefanía, I am also trying to develop a astrocyte-microglia co-culture and I want to use established cell lines and not primary cells. I was thinking about C8-D1A astrocytes and C8-B4 microglia, both available from ATCC. But I am having troubles culturing the C8-B4 so I think I will need to change strategy...
Have you found a solution to this question you asked one year ago?
Thanks, Chiara
Chiara Bastiancich In fact we had decided to do the same as you: C8-B4 and C8-D1A together. We started culturing the C8-B4 but we are not at the point of culturing together yet. Fortunately, I have managed to culture the microglia so far, could you tell me which problems had you encountered while culturing the microglia?
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