Does anybody has the biodot zx1010 manual?

25 January 2021 6,257 1 View

Which microglial cell line will be useful in studying inflammation in the spinal cord?

Most of the studies use primary cells or stem cells for modelling the spinal cord, but as I never worked with these cells, I would like to start with a cell line. Can anybody suggest a good...

07 July 2020 4,248 2 View

Problems with transfecting fibroblasts?

Hello I am writing because I am having serious problems transfecting foreskin human fibroblasts with several plasmids at a time. At first we started using the transfection reagent Trans-IT-2020...

01 April 2019 1,276 4 View

Any experts in Yeast?

Hello, I am looking for somebody with knowledge about S. cerevisiae yeast and petit colonies. Cheers, Estefania

19 August 2018 8,138 5 View

Should I rehydrate frozen pollen before extracting RNA? or before germinating it in-vitro?

14 May 2017 139 3 View

Does anyone have knowledge on Anti-CCL2 antibody?

28 September 2014 7,885 3 View

SW or computational tool to find homologies in animal-plant transcription factors?

Does anyone know if there is any SW or tool available that would allow us to identify transcription factors (TFs) important for our research problem (in plants) by finding similarities among TFs...

16 February 2014 6,464 1 View

MTT assay on 48 well-plate

Hello, everybody, I am performing an experiment with the MG-63 cell line in 3 concentrations: 70,000 cells/well, 60,000 cells/well and 50,000 cells/well, and I want to compare the mtt and mts...

01 January 1970 7,229 3 View

What is the best way to evaluate apoE expression by westernblot in the medium?

I need to evaluate the complete apoe expression under differents tratments but i dont have access to elisa assay to quantify the apoe that been release

01 January 1970 4,085 1 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View