you may use the same approach as been cited in the attached paper

Characterization of a thermotolerant laccase produced by Streptomyces sp. SB086 (Annals of Microbiology, 2014, 64:1363-1369) We have also developed specific primers for detecting laccases genes from actinomycete isolates: Oligonucleotide primers for specific detection of actinobacterial laccases from superfamilies I and K (Antonnie van Leeuwenhoek, 2014, 106:391-398) And if you need further information, you could check the review: Laccases from Actinobacteria—What We Have and What to Expect (Advances in Microbiology Vol.4 No.6(2014), Article ID:45726) Good lucky with your research, Zucchi

Dear Lalita,

Recently I've published a manuscript in which we've used oatmeal agar amended with 0.01% guaiacol for detect laccase from actinomycete:

Dear Tiago ,

Both the links were very helpful thanks.