Mueller-Hinton agar medium is used to assess antibiotic susceptability for bacteria.
when we used this medium for sulfanilamide against Gram positive and Gram negative test strains, no inhibition was observed even at high concentration (100 mg/L).
What is the best way for choosing membership function (MATLAB)
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Genetic Algorithms open source program
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What are the environmental biotech companies (not public culture collections) that could sell/purchase microorganisms with special biodegradative capacities?
27 February 2018 8,304 3 View
Xerophiles
22 September 2014 4,108 2 View
Biodiesel production
17 December 2012 1,757 3 View
02 March 2021 3,060 3 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
28 February 2021 9,936 2 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines
24 February 2021 2,268 3 View