while isolating basidiomyctes from wood , some ascomycetes (trichoderma, Aspergillus) thrive more faster and contaminate the plates, i'm looking for a method or a chemical that slow or inhit the growth of these ascomycetes
You can add 1-10 mg/L of a benzimadazole fungicide like benomyl or thiophanate-methyl or carbendazim to the medium. It inhibits most ascomycetes (except Dothidieomycetes) and some basidiomycetes. Also, 1-2 mg/L dichloran (Botran WP) will inhibit most zygomycetes. Both are autoclavable. Determine sensitivity of target fungi empirically. Add antibiotics as needed.
Benomyl is a good option but it does not inhibit fast-growing Zygomycetes. You can incubate plates at a low temperature (4-10-15ºC) to slow them down and give basidiomycetes better chances to grow.
Hi Tahar
I suggested for you this paper, You can check it and use the medium
This reference:
Article Isolation of Saprophytic Basidiomycetes from Soil
Hello, I suggest using selective media for Basidiomycetes: Mycologia, 83(3), 1991, pp. 296-302.,1991, MEDIA FOR SELECTIVE ISOLATION OF HYMENOMYCETES
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