We prepared alginate beads 2% in CaCl2 200 mM, after stirring for 1h the beads were washed with water, and incubated with the substrate in the presence of 100 mM tartrate buffer pH 5.5 after 1h incubation we noticed huge weight loss of the beads
I think it has something to do with CaCl2 concentration.
High concentration of CaCl2 might cause breakage and weight loss of alginate beads.
I recommend to check this article (open access)
https://doi.org/10.1002/cbic.202300456
I hope you find it helpful.
Best regards.
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