It should be inexpensive and easily available.Wire must not break when i pull the samples with it...
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
Dear All, I am trying to define the dispersion curves of a periodic unit cell in COMSOL Multiphysics with prescribed displacements. I am using two separate studies: - Study 1: Stationary, with...
13 July 2024 7,855 1 View
The reflexes within the measured XRD pattern are not clearly visible or fully indicated, and a legend is missing. explain this.
27 June 2024 7,257 3 View
I have 3 different specimens. Each specimen has a main peak of the targeted phase, however, all of them also have a secondary peak of associated with a secondary phase. Now just based on the...
26 June 2024 5,049 5 View
I isolated my cloned plasmid from TOP10 cells using invitrogen plasmid miniprep kit and eluted it in TE buffer. But I noticed that contrary to my plasmid aliquot, it does not freeze at -20 degree...
19 June 2024 1,612 8 View
Hello! I am pursuing Ph.D. in Environmental Sciences from Sambalpur University, India. My thesis topic broadly focuses on physico-chemical analysis of water and soil samples. I eagerly want to...
18 June 2024 1,897 4 View
I am analyzing the zero field muSR data using kubo-Toyabe function. The fit is not converging. I am noted that the asymmetry term on y-axis is neative beyond certain time. I think this is the...
15 June 2024 2,661 0 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
Hello everyone, without going too much into details, my thesis is about improving the bonding strength at the wire bonding process. I should focus on laser pretreatment of the substrate. Has...
17 June 2024 366 1 View
I am trying to do Grounded Theory in documentary research. I have obtained the most frequent terms (induction) to perform deductive coding and so, articulate a hypothesis about the hidden...
05 June 2024 2,599 2 View
Recently I have an assignment about Simulating magnetic field and I will need the result to derive it. But when i downloaded it i noticed that i am just a new to Simulation software. So can anyone...
04 June 2024 1,270 0 View
Hello. I'm currently working on a control system for a doubly fed induction generator (DFIG) as part of my thesis project. Traditionally, fuzzy logic controllers (FLCs) use the error (e) and the...
27 May 2024 1,732 2 View
Let's consider two windings containing thin diameter wire(0.3 mm) and thick diameter wire(4 mm). These windings operate under the following conditions. 1- The same power is applied. 2- They have...
22 May 2024 7,255 5 View
Hello everyone, I need assistance to remove the oxide formed on the Ti6Al4V. During the cutting by wire EDM, oxide was formed on the surface of the alloy. Since the micron level features are...
22 May 2024 5,871 4 View
Hi, our lab has recently started working with primary hepatocytes in 3D form. The lot we are using has high basal activity which declines as the spheroids age. We are looking to undertake drug...
21 May 2024 1,321 0 View