Arbitrary cut-off values for forest using NDVI?

I'm trying to use NDVI data to define forested land in the southeastern United States. The data is made up of 1m pixels. My goal was to classify each pixel as vegetation or not, then use a cut-off...

22 May 2019 1,251 8 View

Why is my media turning black?

I have a cell line that causes DMEM to turn a dark purple/black colour when the cells are 100% confluent. However, this only happens when the cells are stably transfected with a specific plasmid,...

17 March 2019 3,621 4 View

Literature on not providing care as not wanting to be accountable/take resposibility?

Hi, I'm writing an essay currently on an ethical dilemma were someone i cared for was catatonic. I'm looking for literature aound healthcare professionals not wanting to provide certain types of...

16 February 2018 6,503 3 View

Antibodies for targeting CRF1 and CRF2?

Does anyone known of any antibodies I can use to tag CRF1 and CRF2 receptors?

23 July 2017 8,862 1 View

QPCR Cost Analysis

I'm doing a cost analysis for a PCR experiment and want to make sure I have left anything out that I may need. The list is currently as follows: Primers, TRIzol Reagent, RNAse Free Water, RNAse...

09 July 2017 4,830 4 View

Does anyone know of a good housekeeping gene for gfap and/or Glt1 expression?

In need of a reliable housekeeping gene to normalize my pcr data to. Targeting Gfap and GLT-1 genes.

02 July 2017 567 4 View

How much tissue can I expect to get from a VTA dissection of mice brain?

I'm trying to anticipate how many mice I'll need for RT-qPCR of several genes expressed in the midbrain, specifically the VTA. I need to be able to get about 4mg of VTA tissue from each brain. Is...

29 June 2017 3,703 3 View

What RNA isolation kit would you recommend I use in isolating RNA from brain tissue?

I'm doing RT-qPCR and was wondering which RNA isolation kit I should use.

29 June 2017 8,327 3 View

How do you perform protein/antibody viscosity measurements?

I was wondering for those out there how you currently perform protein viscosity measurements (or antibodies)? What do you use the viscosity measurements for/why do you care about the viscosity?...

27 November 2016 7,490 4 View

What is the fundamental use of Sorensen's glycine buffer?

18 May 2015 6,877 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View