10 October 2017 3 8K Report

Hello, which commercial kit is the best for RNA isolation from laser microdissected FFPE specimens? We need very good quality RNA for further processing (and min. 150ng). Best wishes

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What are the most convincing applications of ontologies and knowledge graphs in science and engineering?

I totally can imagine what a huge potential semantic technologies such as knowledge graphs, ontologies, SPARQL search and automated reasoning might have in different fields of science and...

22 November 2022 578 1 View

Which collections or catalogues of dynamical systems (for control engineering) do you know?

Background: In control theory we often develop new methods for analysis or controller/observer/filter design and usually evaluate them with numerical simulations. In many papers only a few...

06 October 2022 9,980 3 View

What is is the simplest way to setup a local rdf triple store with SPARQL endpoint?

For learning SPARQL it might be useful to have full control over both the query text and the data (RDF triples). While there are many public SPARQL endpoints available their data is typically...

05 March 2022 2,286 3 View

Should a "redundant" subclass relation be present in an OWL ontology after reasoning?

I try to understand the rector-normalization-technique [1] for ontologies in detail. [1] https://douroucouli.wordpress.com/2019/06/29/ontotip-learn-the-rector-normalization-technique/ The...

16 February 2022 2,135 0 View

Which EQ5D value set should be used for multinational study?

Which approach is the more recommended approach in calculate EQ5D values for patients in different country in a normal study (no cost analysis or comparative study between countries)? I want to...

22 May 2018 4,829 3 View

How to separate in vitro differentiated adipocytes from undifferentiated?

Hi, I can differentiate primary white preadipocytes to mature adipocytes in vitro. At day 8 of differentiation I want to separate the cells that differentiated (lipid inclusion) from the...

10 December 2015 2,835 2 View

Can somebody working on Stenotrophomonas help with a quick medical consultation?

9-month old baby after liver transplantation, with multi organ dysfunction, treated already with trimethoprim-sulfamethoxazole

16 October 2014 2,500 5 View

Using Python for Semantic (Web) taks (pros and cons)

The term "Semantic Web" goes back to at least 1999 and the idea – enable machines to "understand" information they process and enable them to be more useful – is much older. But still we do not...

01 January 1970 2,026 6 View

Why is mathematics barely covered by Linked Open Data or other types of semantic technologies?

As it is well known, Linked Opend Data (LOD) and computational ontologies have great success in the fields of Life Sciences (Biology, Medicine, etc). See e.g. the big LS-cluster at . However, I...

01 January 1970 564 7 View

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