Why can I not use mathematic expression with "j" ( complex number) in MathCAD ?

10 November 2018 4,907 1 View

Is N-methacryloyl l-histidine methylester (MAH) still available ?

Hi all scientist I am doing my research work related to polymer, so I need to use N-methacryloyl l-histidine methylester (MAH) chemical. Unfortunately, I can not find any supplier for it. Does...

05 June 2018 8,348 0 View

Why the MIP layer was cracked under UV-exposing ?

Hi everyone I am doing with MIP technique now and, I tried to following to attached paper for MIP chemicals and protocols. To practice MIP, I tried to coat a thin layer of polymer solution on a...

02 March 2018 4,816 0 View

Etching AT-cut type of Quartz Wafer ?

Hi all scientists I am gonna do a etching process with AT-cut Quartz wafer for my QCM sensor. My plate thickness is 160um, and its shape is 200 x 200 mm. Etching area will be circular shape with...

11 December 2017 9,409 0 View

How to double-side mask aligment for 1 inch wafer ?

Hi all I need to fabricate my device on quartz wafer with 1 inch in diameter and both electrodes on top and bottom side must be high concentric. So I need do this work on double-side mask...

09 October 2017 181 0 View

How to define the Quartz material in ANSYS ?

Hi Scientists I gonna do a simulation for my device ( with Quartz material, AT-cut type), using ANSYS. But I dont know how to create or define this material in ANSYS ? Hence, I post this my...

09 October 2017 5,644 0 View

Implementation of PDE equation in COMSOL ?

08 September 2017 6,808 0 View

Can I apply Matthieu equation for the rectangular plate?

Hi Scientist I am doing a design my sensor based on the rectangular quartz plate. I reviewed some paper about introduced Mathieu equation for analysing the vibration of the plate, but all plate in...

08 September 2017 3,890 0 View

Which type of OTA have "negative transconductance " ?

08 September 2017 7,803 11 View

Help me to clear problem in frequency domain (COMSOL) ?

Hi all I have a problem with eigenfrequency and frequency domain, I hope someone can help me to clear I using PDE Coefficient form, and solve my problem in 2D, with independent variable (u) =...

08 September 2017 202 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View