Why can I not use mathematic expression with "j" ( complex number) in MathCAD ?

10 November 2018 4,907 1 View

Which is type of "selective switch valve" for microfluidic application ?

Hi all experienced scientists I am doing a sensing measurement based on microfluidic device. As I sketched in my attached file, I have one buffer solution (PBS) and nearly 8 bacteria samples....

07 August 2018 7,478 0 View

Is N-methacryloyl l-histidine methylester (MAH) still available ?

Hi all scientist I am doing my research work related to polymer, so I need to use N-methacryloyl l-histidine methylester (MAH) chemical. Unfortunately, I can not find any supplier for it. Does...

05 June 2018 8,348 0 View

Why the MIP layer was cracked under UV-exposing ?

Hi everyone I am doing with MIP technique now and, I tried to following to attached paper for MIP chemicals and protocols. To practice MIP, I tried to coat a thin layer of polymer solution on a...

02 March 2018 4,816 0 View

Etching AT-cut type of Quartz Wafer ?

Hi all scientists I am gonna do a etching process with AT-cut Quartz wafer for my QCM sensor. My plate thickness is 160um, and its shape is 200 x 200 mm. Etching area will be circular shape with...

11 December 2017 9,409 0 View

How to double-side mask aligment for 1 inch wafer ?

Hi all I need to fabricate my device on quartz wafer with 1 inch in diameter and both electrodes on top and bottom side must be high concentric. So I need do this work on double-side mask...

09 October 2017 181 0 View

How to define the Quartz material in ANSYS ?

Hi Scientists I gonna do a simulation for my device ( with Quartz material, AT-cut type), using ANSYS. But I dont know how to create or define this material in ANSYS ? Hence, I post this my...

09 October 2017 5,644 0 View

Implementation of PDE equation in COMSOL ?

08 September 2017 6,808 0 View

Which type of OTA have "negative transconductance " ?

08 September 2017 7,803 11 View

Help me to clear problem in frequency domain (COMSOL) ?

Hi all I have a problem with eigenfrequency and frequency domain, I hope someone can help me to clear I using PDE Coefficient form, and solve my problem in 2D, with independent variable (u) =...

08 September 2017 202 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View