Which is the best way to visualize CpG methylation percentage from pyrosequencing ?

More Spiros Rigas's questions See All

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Hi, If I want to measure cancer cell death after a protein-drug treatment which FACS protocol should i use? Annexin V staining ,PI staining or both?

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Simon Neil Jarman

That depends on your question to some extent, but there may be biological significance to the individual CpG site GC%, so best to analyse them individually as the first step. An overall mean value is then easy to derive from that, which may have a slightly different biological significance. With that approach you don't lose any information at any step.

Spiros Rigas

Thank you so much Simon for your response. I was at the similar direction, about the utility of mean values, but not 100% sure.