You could try testing it but considering that your antibody was frozen without any stabilizer like glycerol it probably lost its function. Another problem is that Phycobiliproteins like PE are sensitive to freeze thaw cycles.
yes but it was frozen before 1 month and we realize it now so there werent any freeze thaw cycles because i havent used it yet
Answer this question is quiet difficult, propably not even the manufacturer will be able to answer it because it depends on the fluorochrome, the antibody and the instrument you use. The problem is that only a part of your conjugated has degraded but to determine the extension of this degradation you will need to check it experimentally. If your antibody was raised against a high density antigen chances are you will still be able to detect it. If your antibody recognizes a low density antigen the chances of detection decreases (you will need higher concentrations of antibody). I suggest you choose a cell line or experimental condition that knowingly express your antigen and titrate your antibody. Be careful to choose a couple of concentrations points higher than recommended by the manufacturer. Per example, if the data sheet recommends a 1:250 dilution you should begin at 1:50 and go for a 2fold dilution. Luckly PE is a bright fluorochrome and I believe your antibody will still show a good fluorescence. Also IgG molecules are quiet stable (some Abcam western blot antibodys are kept frozen at -80oC) so a good portion of the molecules will still recognize your antigen. I'm sorry I can't give you a "yes or no" answer.
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