Thank you very much. Ley me try these suggestions.

Could you be a little more specific where the inflammation takes place? 

Is it a superficial and skin inflammation, an inflammation of the meninges or just a plain gliotic respsonse to your patch electrode? Either will need a different approach - besides the sugestions above.

Hi,

Thank you very much for the kind reply. I am doing my experiment in layer 2/3 neocortex. After the experiment I used to cover it with silicon. Still you can see an entire damage from Superficial cranitomy till layer 4-5. I felt it is because I use many pipettes, and then I reduced it into even single pipette, and again there was inflammation!