Arjun A Bhaskaran

10 Questions 17 Answers 0 Followers

Questions related from Arjun A Bhaskaran

Is it possible to calculate Mean Amplitude of EPSPs in Clampfit?

Hi all, I would like to analyse trial by trial mean amplitude of sensory stimulus evoked EPSP responses (40 trials). For the moment I have peak amplitude. But I would like to know if it is...

22 February 2019 852 1 View

What is behind the long silence of Neuron after an evoked response?

I performed a few sensory stimulation experiments in thalamus and recorded responses of pyramidal neurons in cortex using whole cell patch clamp in vivo. Interestingly, I found that just after...

20 August 2018 4,941 6 View

Can anyone suggest some standard Neuronal activity markers?

I would like to mark the cells which respond to different stimuli.Any suggestions? Literature details also will be helpful. Thank you in advance

13 September 2017 5,112 4 View

Welch's unpaired t-test or student's unpaired t-test?

I need to compare two groups with equal /unequal variances. Which t-test is the best? If I am using Welch's unpaired t-test, should I do an 'F-test two-sample for variances' first and see if the...

25 July 2017 3,191 4 View

Can anyone suggest a high speed video camera?

Please suggest me a "cheaper" but high speed video camera (Company & model) for whisker movement tracking. Thank you

30 March 2016 9,328 5 View

Anyone knows a protocol for a blind single cell electroporation in vivo for gene transfer?

I am trying some in vivo electroporation for a plasmid transfection. Most of the papers that I am going through is two photon guided electroporation protocols! Does anyone know a protocol which...

08 January 2016 4,236 3 View

Does a plasmid influence the Osmolarity of intracellular solution?

Will addition of plasmid can modify the osmolarity of an intracellular solution for in vivo? Eg: Osm of intra (1x) is 290mm. will it increase, decrease or null the osm of the final...

03 September 2015 6,457 4 View

How can I avoid AgCl crumbling?

How can I avoid AgCl crumbling of recording electrode (patch clamp). I tried to replace the silver wire and chlorinate using KCl (2M). But when ever I try to patch, small black and silver...

30 August 2015 3,661 9 View

How can I make 1M KMeSO3 stock solution without having precipitation?

I wanted a 1M stock solution of KMeSO3 for my intracellular solution. It is not diluting completely! Any idea? Thanks in advance :)

12 August 2015 5,762 2 View

Which is the best way to avoid an inflammation?

Hi, I am doing Patch clamp, in vivo. Many times I am encountering an inflammation at the site of patch after a day or two!! Can anyone help me with a good solution to avoid inflammation? As I am...

06 August 2015 2,946 3 View