i think it's depend on your aim that commercial kits typically has more traceable sample with low amount of super-coil, however chloroform procedure has inverse result.
The best protocol for genomic DNA extraction from bacteria depends on the specific bacterial strain and the downstream application, but some general guidelines such as adequate cell lysis and careful control of pH and temperature can help to ensure an efficient and high-quality extraction.
biorad Finpipeppette genomic DNA isolation kit use molecular grade TAE, TE, TRIS Buffer reagents and centrifuge 3-5 times for better result with reagents
From my experience, the quality, purity, and amount of the DNA are usually superior when isolated via specialized kits. Various methods yield different fragment lengths and different DNA concentrations which are both critical for nanopore sequencing. Because of this, the suitability of different methods depends on what you are trying to achieve with nanopore sequencing.
I would say that the specialized kits are really worth buying, as the results are better, but I was also successful using CTAB extraction with some additional purification steps.
The best protocol for efficient and good quality genomic DNA extraction from bacteria is the cetyltrimethyl ammonium bromide (CTAB) protocol. This protocol uses a combination of detergents, salts, and enzymes to lyse the bacterial cell wall and separate the DNA from other cellular components. The DNA extracted using this method is of high quality and is suitable for various downstream applications such as PCR, sequencing, and restriction digestion.
The best protocol for efficient and high-quality genomic DNA extraction from bacteria is the High Pure PCR Template Preparation Kit from Roche. This kit uses a combination of the alkaline lysis and chaotropic salt methods to isolate pure, high-quality DNA from bacteria, resulting in reliable and reproducible results.
There are several protocols available for the extraction of genomic DNA from bacteria, and the best one depends on the type of bacteria, the intended downstream application, and the available resources. A commonly used and efficient protocol for bacterial genomic DNA extraction is the CTAB (cetyltrimethylammonium bromide) method.
the best method via which i get sufficient and good amount of gDNA from bacterial cells are follow;
after centrifugation TE buffer and SDS were added to the cell followed by one hour of incubation. then add CTAB /NaCl solution and keep for 10 minutes. now add same volume of chloroform/isoamyl alcohol and centrifuge. collect the supernatant and add phenol/chloroform/iso amyl alcohol. take the supernatant and add isopropanol (chilled). again centrifuge and then washed twice with 70% ethanol. keep it room temperature to evaporate the ethanol and add 100miorliter of TE buffer.