Priyanka Basak

6 Questions 1 Answers 0 Followers

Questions related from Priyanka Basak

Facing a syntax error problem while running BRIG with genbank file what's the solution?

I am facing a problem while running BRIG with genbank files. I have given plasmid sequences as my input files. I want to give gene annotations & following the manual I have added the genes...

06 August 2023 808 0 View

Facing a syntax error problem wile running brig with genbank file what's the solution?

I am facing a problem while running brig with genbank files. I want to give gene annotations & following the manual I have added the genes with the start & stop codon mentioned. But after...

06 August 2023 5,601 0 View

Facing a problem to circularize the bacterial plasmid during hybrid assembly in unicycler. What's the solution?

I am facing a problem to circularize a particular bacterial plasmid sequence while doing hybrid assembly in unicycler. After the assembly, it's not showing circular. While the other plasmids have...

30 July 2023 1,250 1 View

I have isolated Gram-negative bacterial genomic DNA using promega wizard DNA extraction kit for nanopore sequencing. Why there are smears in my gel?

I have isolated genomic DNA from Klebsiella pneumoniae using promega wizard bacterial genomic DNA extraction kit. I have followed the protocol instructions. But I can see smears in my gel and...

14 April 2023 8,399 2 View

Which is the best protocol for the efficient & good quality Genomic DNA extraction from bacteria?

Which is the best protocol for the good quality genomic DNA extraction from the bacteria for nanopore sequencing other than commercial kits.?

14 April 2023 7,878 8 View

If pMLST is different within for example IncFII plasmid replicon, will the whole plasmid environment will be similar or dissimilar?

Suppose I want to see the plasmid similarities within IncFII replicon type carrying NDM-5 for example in E. coli isolates, but my pMLST of IncFII replicon is different within these isolates, will...

01 January 1970 6,222 2 View