All the experimental results need to be presented with statistical analysis. In case of adsorption isotherm and kinetic study, what does I include?

What are the things should I include under statistical analysis.

19 November 2020 5,098 2 View

How to clone a promoter region of a gene within pBI101.2 vector for GUS assay?

I am cloning about 1.5kb promoter of a gene within pBI101.2 vector for GUS assay. During the primer designing, I am facing a problem. Should I include some portion(say about +20 to +30 from the...

10 June 2019 6,950 2 View

Need a protocol for SOD, Catalase and LPO enzyme assay in 96 well plate?

I need detailed protocol. Thanks in advance.

20 June 2018 6,887 3 View

What are the methods to follow if I want to study cross talk between molecular pathways?

If I want to study the cross talks between pathways involving more than 20-30 genes, what are the methods I should follow? qRT PCR and then validation by Western Blot or Immunohistochemistry is...

19 June 2018 7,134 2 View

No title

19 June 2018 6,121 3 View

How to plot in-plane vibration modes of square plate using MATLAB. The plot looks like a distrorted X-Y plane grid ?

I have the natural frequencies and the related displacement equations in x, y directions for in-plane vibration. Every point in the plate has a displacement in x (denoted by u(x,y) ) and y...

30 October 2017 304 11 View

How to check purity of mono-/bis-/metal complex-azo dyes using UV-Vis Spectroscopy?

How to check purity of mono-/bis-/metal complex-azo dyes using UV-Vis Spectroscopy?

22 October 2017 3,767 6 View

Given a bare-Lagrangian for a renormalizable, interacting system, is the renormaized version unique? If yes, then which ?

It is well-known that several renormalized versions of a given theory may emerge depending upon the chosen regularization method, choice of subtraction scale/scheme,...

09 March 2017 8,192 40 View

Perovskites are a class of compounda, than how can we identify that which material is suited for harvesting solar energy?

26 February 2017 4,620 3 View

Between ER and SQ grade reagents of Qualigens (Thermo fisher) which one is analytical grade [AR] reagent ?

23 November 2016 716 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View