Rekha Garg

5 Questions 6 Answers 0 Followers

Questions related from Rekha Garg

Which is the best kit/enzymatic method Efficient for the isolation of cells from the mouse or human tumors?

I want to isolate the cells from the tumors (mouse model and human). I read that Collagenase I is harsher than Collagenase IV. Can anyone please suggest if there is any commercial kit with the...

09 August 2018 5,066 2 View

Is it okay to infect THP-1 monocytes with virus without PMA differentiation?

I am infecting THP-1 cells with virus in my studies without PMA stimulation. I am not sure if it is accepted scientifically. Please suggest.

20 December 2017 4,622 2 View

How can I measure concentration of antiVirus IgA in mouse serum?

Hi, Is there any way to calculate actual concentration of antivirus IgA/ IgG/IgM in mouse serum using ELISA method? Can I use any indirect method for this, e.g. using mouse IgA purified protein as...

23 July 2015 4,841 2 View

What are other markers for gamma delta T cells in the intestinal region?

Dmitry and Ali, can you please help me to know if gamma delta T cells in the gastrointestinal tract have other specific marker or specific cytokine secretion other than regular CD8 or CD4 T cells?

22 April 2015 2,375 1 View

Does anyone know if gammadelta CD8 cells act as tissue resident memory T cells in mice?

I need to know if CD8 gamma delta T cells are considered as Tissue resident memory T cells and they produce interferon/granzyme in wild type mice too?

17 April 2015 8,649 5 View