Which is the best and dual cell line for EGFR and HER2.?

09 April 2022 1 9K Report

For evaluation of anticancer activity via MTT assay and Enzyme inhibition assay for the synthesized small molecules which cell line is best for EGFR and HER2 protein in the breast cancer.

Malcolm Nobre

Hello Prasad Dhiwar

You may use BT474 and SKBR3 cell lines. Both are breast cancer cell lines expressing high level of HER2 and a decent level of EGFR.

Please refer to the research article attached below for more details. See fig 1B.

https://www.cell.com/molecular-therapy-family/nucleic-acids/pdf/S2162-2531(17)30316-5.pdf

Good Luck.

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