Which is a very successful procedure for extraction of genomic DNA from herbarium sample?

More Swetha Balakrishnan's questions See All

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How to give port for printed log periodic dipole array antenna in CST?

PLPDA have radiating element on both sides of the substrate. so how to apply port. i have attached the figure below.

03 January 2021 4,005 6 View

I want to design a printed log periodic dipole array antenna on only one side of the substrate. is this acceptable one ?

actually for PLPDA, it is designed on both sides of the substrate for providing 180 degree phase shift between the elements

28 December 2020 3,148 3 View

How to isolate the pure Ruthenium DMSO complexes from the reaction mixture?

The reaction between N-S bidentate/ONS tridendate ligand (TSC) and cis[RuCl2(DMSO)4] in ethanol by reflux is complete as seen from TLC chromatography. But removing the excess solvent through...

27 December 2020 6,630 1 View

What may be the reasons for broad spectrum in proton NMR (taken in DMSO-d6) for Pd(II) complexes?

The synthesized Pd(II) complexes involved -NH group attached to C=S moiety. The 1H proton NMR taken in DMSO-d6 solvent consisted of a very broad peak at 4.8 ppm with an integration of 6. They are...

09 December 2020 4,340 3 View

What may be the reasons for broad spectrum at 4.8 ppm in proton NMR (taken in DMSO-d6) for Pd(II) complexes?

The synthesized Pd(II) complexes involved -NH group attached to C=S moiety. The 1H proton NMR taken in DMSO-d6 solvent consisted of a very broad peak at 4.8 ppm with an integration of 6. They are...

09 December 2020 9,960 3 View

How to change the protein chain names X into A, B, C, D after MD simulations in Gromacs?

I am running MD simulations for holoenzyme in Gromacs. The protein has four chains like A, B, C, D. After MD simulations, All the chains are showing as X chain in VMD and even in the PDB file....

12 November 2020 6,228 1 View

I am electroplating Ni on a stainless steel. At temperature and time should I anneal so that Ni thermally diffuses into stainless steel?

I am electroplating Ni on a stainless steel for abrasive application. At temperature and time can I anneal so that Ni thermally diffuses into stainless steel? My intention is to improve the...

20 October 2020 7,092 3 View

How to visualize cells seeded on Millicell-HA transwell?

Hi, I am working with Millicell-HA transwell to culture HCE-2 cells. But as the transwell in opaque, not able to visualize the cells. Can someone help me? Thanks in advance.

14 November 2019 3,573 1 View

Can anyone help me to get all the variables imported in NLogit?

While i try to import the data from excel files to NLogit 4, only 18-19 variables being imported and the rest 6-7 variables is missing in the imported list. I have tried in changing the memory...

12 November 2019 9,760 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Arvind Singh

Please have a look at these useful PDF attachments.

Oadi Matny

Hi Swetha: Find the link for useful reference:

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.467.7933&rep=rep1&type=pdf

http://www.phytoneuron.net/2014Phytoneuron/48PhytoN-DNAextraction.pdf

https://link.springer.com/chapter/10.1007%2F978-1-4612-4318-2_11

http://www.biotechniques.com/BiotechniquesJournal/2017/February/Extraction-of-ultrashort-DNA-molecules-from-herbarium-specimens/biotechniques-365631.html